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You have water inside your system, which is being pulled into the cold trap and freezing. Please check your solvents to make sure they were not left open and that you are using the correct grade reagents. Make sure the scientific glassware is as dry as possible by using a desiccator or an oven to dry your glassware. Check for vacuum leaks as moisture may be leaking into the system.

Specific temperatures will require different mixtures of varying substances to achieve certain set points. It is recommended that you check your owner's manual first for suggestions.

Please refer to your owner's manual that came with your chiller.